Study Materials for Molecular Techniques
PCR and Gel Electrophoresis
- How PCR (polymerase chain reaction) works [YouTube]
- Taq polymerase and general PCR guide [NEB]
- How to design primers manually [BioWeb Home]
- Gel electrophoresis (How to make gel, and run electrophoreisis) [YouTube]
- Gel electrophoresis (Principle) [YouTube]
- Materials: Platinum Taq Polymerase, for general use [Life Tech] High Fidelity Taq Polymerase, for DNA barcoding [Life Tech] GelStar, for gel staining [LONZA] GelRed, for gel staining [Biotium]
Gel Extraction and Cloning
- How TA cloning works [YouTube Video]
- Materials
QIAquick PCR Purification Kit [Qiagen]
Gel Extraction Kit, QIAEX II [Qiagen]
TOPO TA Cloning Kit [Invitrogen]
pGEM T Easy Vector [Promega]
Competent Cells, subcloning efficiency [Life Tech]
Plasmid DNA extraction kit [QIAprep mini kit, Qiagen]
Sequencing
- How Sequencing reaction works, Sanger method [YouTube]
- UCDavs Sequencing Facility [Click HERE]
- Davis Sequencing [NUCLEICS]
RNA isolation methods
- TRIZOL Reagent [Life Tech]
Assess quality of RNA samples
cDNA synthesize
Quantitative (RealTime) PCR
- How qPCR assay works (Probe method, absolute copy number) [YouTube]
- How qPCR assay works (Sybr Green, gene expression analysis) [ YouTube]
- UCDavis Real-Time PCR Research and Diagnostics Core Facility (TaqMan Lab): Click HERE for the top page. Instruction for External Run.
RNA in situ hybridization
- RNA in situ hybridization: Click HERE
Next Generation Sequencing, Illumina platform
RNA-Seq
- RNA-seqlopedia [Cresko Lab]
ELISA
- Overview of ELISA [ThermoFisher]
- How ELISA works: competitive assay [YouTube]
- Antibody Basics [OpenWetWare]
LAMP Assay
- Loop Mediated Isothermal Amplification (LAMP) Tutorial [YouTube]
- Loop Mediated Isothermal Amplification [PREMIERBiosoft]
- PrimerExplorer [FUJITSU Limited]
